Isiphazamisi seseli ithuluzi lokuhlola elivame ukusetshenziswa elisetshenziselwa ukuhlukanisa amaseli ebhayoloji nokukhulula izinto ezingaphakathi kweseli.Isimiso sokusebenza se-cell breaker sisekelwe esimisweni sokuphuka komzimba kanye ne-oscillation yemishini, futhi inhloso yokuphulwa kweseli ifinyelelwa ngokunikeza amandla anele ukucekela phansi ukwakheka kwamaseli.
Umgomo wokusebenza wesiphazamisi seseli uzokwethulwa ngokuningiliziwe ngezansi.Izingxenye eziyinhloko zesiphazamisi seseli zihlanganisa isilawuli sejubane, igumbi elichotshozayo, ibhola elichotshozwayo kanye nepayipi lesampula, njll. Phakathi kwazo, isilawuli sesivinini sisetshenziselwa ukulawula isivinini sokujikeleza kwegumbi lokuchoboza, okuyisitsha sokugcina. amasampula namabhola agxobayo, futhi amabhola ahlabayo aphula amaseli ngokushayisana namasampula.Ngaphambi kokusebenzisa isiphazamisi seseli, indawo ephazamisayo efanele kufanele ikhethwe kuqala.Imidiya yokuchoboza evame ukusetshenziswa ubuhlalu bengilazi, ubuhlalu bensimbi kanye nobuhlalu be-quartz.
Okucatshangelwayo okuyinhloko ekukhetheni i-medium yokuchoboza uhlobo lwesampula kanye nenjongo yokuchotshozwa.Isibonelo, kumaseli abuthakathaka, ubuhlalu bengilazi obuncane bungasetshenziswa ekuphazamiseni;kumaseli anzima kakhulu, ubuhlalu bensimbi obuqinile bungakhethwa.Phakathi nenqubo yokuchoboza, faka isampula ukuze ichotshozwe emgqonyeni wokuchoboza, bese wengeza inani elifanele lokuchoboza okuphakathi.Khona-ke, isivinini sokujikeleza segumbi lokuchoboza silawulwa isilawuli sesivinini, ukuze i-medium yokuchoboza kanye nesampula ibe nokushayisana okuqhubekayo kwemishini.Lokhu kungqubuzana kungaphazamisa ukwakheka kweseli ngokudluliswa kwamandla, ukuhlakaza ulwelwesi lwamaseli nama-organelles, nokukhulula izinto ezingaphakathi kweseli.
Inqubo yokusebenza yesiphazamisi seseli ikakhulukazi ihilela izici ezibalulekile ezilandelayo: isivinini sokujikeleza, ubukhulu kanye nokuminyana kwendawo yokuchoboza, isikhathi sokuchoboza kanye nezinga lokushisa.Esokuqala isivinini sokujikeleza.Ukukhethwa kwesivinini sokuzungezisa kudinga ukulungiswa ngokuya ngezinhlobo zamaseli ezahlukene kanye nezakhiwo zesampula.
Ngokuvamile, kumaseli athambile, isivinini esiphezulu sokujikeleza singakhethwa ukuze kwandiswe imvamisa yokushayisana futhi ngaleyo ndlela kuphazamise amaseli ngokuphumelelayo.Kumaseli aqinile, njengoba eqine kakhulu, isivinini sokujikeleza singancishiswa ukuze kuncishiswe ukuphazamiseka kwesampula.
Okwesibili ubukhulu kanye nokuminyana kwendawo yokuchoboza.Ubukhulu nobuningi be-medium yokuchoboza kuzothinta ngqo umphumela wokuchoboza.Imidiya encane ephazamisayo inganikeza amaphuzu amaningi okushayisana, okwenza kube lula ukuphazamisa izakhiwo zamaselula.Imidiya emikhulu yokuchoboza idinga isikhathi eside sokuchotshozwa.
Ukwengeza, ukuminyana kwendawo yokuchoboza kuzophinde kuthinte amandla okungqubuzana, ukuminyana okuphezulu kakhulu kungaholela ekuhlukaneni ngokweqile kwesampula.Isikhathi sokuphazamiseka siyipharamitha ebalulekile yokuphazamiseka kweseli.Ukukhethwa kwesikhathi sokuchoboza kufanele kunqunywe ngokusho kohlobo lwesampula kanye nomphumela wokuchoboza.Ngokuvamile, isikhathi eside sokuphazamiseka, yilapho amaseli ephazamiseka kakhulu, kodwa futhi angabangela umonakalo kwezinye izingxenye zesampula.Okokugcina ukulawula izinga lokushisa.Umthelela wezinga lokushisa ekuhlukaneni kweseli awukwazi ukuzitshwa.Ukushisa okuphezulu ngokweqile kungabangela ukuhlukaniswa kwamaprotheni nama-nucleic acid kumaseli, ngaleyo ndlela kuthinte umphumela wokuhlukana.Ngakho-ke, kunconywa ukwenza ukuphazamiseka kweseli ngaphansi kwezimo ze-cryogenic, ezingancishiswa ngokusebenzisa i-chiller noma ukusebenza eqhweni.
Iziphazamisi zamaseli zidlala indima ebalulekile ocwaningweni lwebhayoloji.Ngokulawula ngokunengqondo amapharamitha afana nesivinini sokujikeleza, usayizi kanye nokuminyana kokuchotshozwa okuphakathi, isikhathi sokuchoboza nezinga lokushisa, ukuchotshozwa okusebenzayo kwamaseli kungafinyelelwa.Ngemva kokuba amaseli ephukile, izinhlobo ezihlukahlukene zezinto ezisemangqamuzaneni zingatholakala, njengamaprotheni, ama-nucleic acid, ama-enzyme, njll, okuhlinzeka ngesisekelo esibalulekile sokuhlaziywa nokucwaninga okulandelayo.Ngamafuphi, isiphazamisi seseli iyithuluzi lokuhlola elibalulekile, futhi isimiso saso sokusebenza sisekelwe kumgomo wokuphuka ngokomzimba kanye nokudlidliza komshini.Ukuphazamiseka okusebenzayo kwamaseli kungafinyelelwa ngokulawula imingcele ehlukene njengesivinini sokujikeleza, usayizi nokuminyana kwendawo yokuphazamiseka, isikhathi sokuphazamiseka nezinga lokushisa.Isiphazamisi seseli sisetshenziswa kakhulu, sinikeza lula nokusekelwa kubacwaningi ocwaningweni oluhlobene emkhakheni webhayoloji.
Isikhathi sokuthumela: Sep-06-2023